• Immunofluorescence analysis of U2OS cells using GLS2 Rabbit polyclonal antibody (STJ118469) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GLS2 antibody (STJ118469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GLS2 antibody (545-602) (STJ118469)

SKU:
STJ118469

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLS2 (545-602) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLS2
Gene ID: 27165
Uniprot ID: GLSL_HUMAN
Immunogen Region: 545-602
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 545-602 of human GLS2 (NP_037399.2).
Immunogen Sequence: NPFAKDRWGNIPLDDAVQFN HLEVVKLLQDYQDSYTLSET QAEAAAEALSKENLESMV
Tissue Specificity Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Function Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Protein Name Glutaminase Liver Isoform - Mitochondrial
Gls
L-Glutaminase
L-Glutamine Amidohydrolase
Database Links Reactome: R-HSA-210500
Reactome: R-HSA-5628897
Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glutaminase Liver Isoform - Mitochondrial antibody
Anti-Gls antibody
Anti-L-Glutaminase antibody
Anti-L-Glutamine Amidohydrolase antibody
Anti-GLS2 antibody
Anti-GA antibody

Information sourced from Uniprot.org

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