• Western blot analysis of extracts of various cell lines, using GLP2R antibody (STJ28685) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using GLP2R rabbit polyclonal antibody (STJ28685) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GLP2R antibody (460-553) (STJ28685)

SKU:
STJ28685

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLP2R (460-553) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLP2R
Gene ID: 9340
Uniprot ID: GLP2R_HUMAN
Immunogen Region: 460-553
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-553 of human GLP2R (NP_004237.1).
Immunogen Sequence: SGCRACVLGKDFRFLGKCPK KLSEGDGAEKLRKLQPSLNS GRLLHLAMRGLGELGAQPQQ DHARWPRGSSLSECSEGDVT MANTMEEILEESEI
Function This is a receptor for glucagon-like peptide 2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Protein Name Glucagon-Like Peptide 2 Receptor
Glp-2 Receptor
Glp-2-R
Glp-2r
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Glucagon-Like Peptide 2 Receptor antibody
Anti-Glp-2 Receptor antibody
Anti-Glp-2-R antibody
Anti-Glp-2r antibody
Anti-GLP2R antibody

Information sourced from Uniprot.org

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