| Host: |
Rabbit |
| Applications: |
IF/ICC/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Gli2 |
| Gene ID: |
14633 |
| Uniprot ID: |
GLI2_MOUSE |
| Immunogen Region: |
327-442 |
| Specificity: |
Recombinant Protein corresponding to a sequence within amino acids 327-442 of mouse GLI2 (NP_001074594.1). |
| Immunogen Sequence: |
LTSISTMPTQLSSSSSNCLN DANQNKQNSESAVSSTVNPI TIHKRSKVKTEAEGLRPASP LGLTQEQLADLKEDLDRDDC KQEAEVVIYETNCHWADCTK EYDTQEQLVHHINNEH |
| Post Translational Modifications | Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2.this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2. Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy. |
| Function | Functions as a transcription regulator in the hedgehog (Hh) pathway. Functions as a transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway. Required for normal skeleton development. |
| Protein Name | Zinc Finger Protein Gli2Tax Helper Protein |
| Database Links | Reactome: R-MMU-5610787Reactome: -MMU-5632684 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionCiliumStk36 Promotes Translocation To The NucleusIn KeratinocytesIt Is Sequestered In The Cytoplasm By SufuIn The Absence Of SufuIt Translocates To The Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger Protein Gli2 antibodyAnti-Tax Helper Protein antibodyAnti-Gli2 antibodyAnti-Thp antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
