• Western blot analysis of extracts of various cell lines, using GLI2 antibody (STJ119206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L-929 cells using GLI2 antibody (STJ119206) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (STJ119206) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (STJ119206) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GLI2 antibody (STJ119206)

SKU:
STJ119206

€211.88 - €561.88
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLI2 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Gli2
Gene ID: 14633
Uniprot ID: GLI2_MOUSE
Immunogen: Recombinant protein of mouse GLI2.
Immunogen Sequence: Email for sequence
Post Translational Modifications Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2.this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2. Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.
Function Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway. Required for normal skeleton development.
Protein Name Zinc Finger Protein Gli2
Tax Helper Protein
Database Links Reactome: R-MMU-5610787
Reactome: -MMU-5632684
Cellular Localisation Nucleus
Cytoplasm
Cell Projection
Cilium
Stk36 Promotes Translocation To The Nucleus
In Keratinocytes
It Is Sequestered In The Cytoplasm By Sufu
In The Absence Of Sufu
It Translocates To The Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein Gli2 antibody
Anti-Tax Helper Protein antibody
Anti-Gli2 antibody
Anti-Thp antibody

Information sourced from Uniprot.org

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