| Host: | Rabbit |
| Applications: | IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Gli2 |
| Gene ID: | 14633 |
| Uniprot ID: | GLI2_MOUSE |
| Immunogen Region: | 327-442 |
| Specificity: | Recombinant Protein corresponding to a sequence within amino acids 327-442 of mouse GLI2 (NP_001074594.1). |
| Immunogen Sequence: | LTSISTMPTQLSSSSSNCLN DANQNKQNSESAVSSTVNPI TIHKRSKVKTEAEGLRPASP LGLTQEQLADLKEDLDRDDC KQEAEVVIYETNCHWADCTK EYDTQEQLVHHINNEH |
| Post Translational Modifications | Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2.this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2. Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy. |
| Function | Functions as a transcription regulator in the hedgehog (Hh) pathway. Functions as a transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway. Required for normal skeleton development. |
| Protein Name | Zinc Finger Protein Gli2 Tax Helper Protein |
| Database Links | Reactome: R-MMU-5610787 Reactome: -MMU-5632684 |
| Cellular Localisation | Nucleus Cytoplasm Cell Projection Cilium Stk36 Promotes Translocation To The Nucleus In Keratinocytes It Is Sequestered In The Cytoplasm By Sufu In The Absence Of Sufu It Translocates To The Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger Protein Gli2 antibody Anti-Tax Helper Protein antibody Anti-Gli2 antibody Anti-Thp antibody |
Information sourced from Uniprot.org