• Immunofluorescence analysis of L929 cells using GK2 Rabbit polyclonal antibody (STJ119588) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GK2 Rabbit polyclonal antibody (STJ119588) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GK2 Rabbit polyclonal antibody (STJ119588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-GK2 antibody (1-150) (STJ119588)

SKU:
STJ119588

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GK2
Gene ID: 2712
Uniprot ID: GLPK2_HUMAN
Immunogen Region: 1-150
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GK2 (NP_149991.2).
Immunogen Sequence: MAAPKTAAVGPLVGAVVQGT NSTRFLVFNSKTAELLSHHK VELTQEFPKEGWVEQDPKEI LQSVYECIARTCEKLDELNI DISNIKAVGVSNQRETTVIW DKLTGEPLYNAVVWLDLRTQ TTVEDLSKKIPGNSNFVKSK TGLPLSTYFS
Tissue Specificity Testis-specific. Expressed in the midpiece of spermatozoa.
Function Key enzyme in the regulation of glycerol uptake and metabolism. Essential for male fertility and sperm mitochondrial sheath formation. Required for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during spermatogenesis. Can induce mitochondrial clustering through interactions with PLD6 and up-regulation of phosphatidic acid synthesis in the mitochondria.
Protein Name Glycerol Kinase 2
Gk 2
Glycerokinase 2
Atp -Glycerol 3-Phosphotransferase 2
Glycerol Kinase - Testis Specific 2
Database Links Reactome: R-HSA-75109
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasm
In Sperm The Majority Of The Enzyme Is Bound To Mitochondria
Alternative Antibody Names Anti-Glycerol Kinase 2 antibody
Anti-Gk 2 antibody
Anti-Glycerokinase 2 antibody
Anti-Atp -Glycerol 3-Phosphotransferase 2 antibody
Anti-Glycerol Kinase - Testis Specific 2 antibody
Anti-GK2 antibody
Anti-GKP2 antibody
Anti-GKTA antibody

Information sourced from Uniprot.org

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