Western blot analysis of various lysates using GINS2 Rabbit pAb (STJ111598) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GINS2 antibody (1-185) (STJ111598)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111598
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General Information

Short DescriptionRabbit polyclonal GINS2 (1-185) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGINS2
Gene ID51659
Uniprot IDPSF2_HUMAN
Immunogen Region1-185
Immunogen SequenceMDAAEVEFLAEKELVTIIPN FSLDKIYLIGGDLGPFNPGL PVEVPLWLAINLKQRQKCRL LPPEWMDVEKLEKMRDHERK EETFTPMPSPYYMELTKLLL NHASDNIPKADEIRTLVKDM WDTRIAKLRVSADSFVRQQE AHAKLDNLTLMEINTSGTFL TQALNHMYKLRTNLQPLEST QSQDF
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human GINS2 (NP_057179.1).

Additional Info

Function Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
Protein Name Dna Replication Complex Gins Protein Psf2
Gins Complex Subunit 2
Database Links Reactome: R-HSA-176974
Cellular Localisation Nucleus
Chromosome
Associates With Chromatin
Alternative Antibody Names Anti-Dna Replication Complex Gins Protein Psf2 antibody
Anti-Gins Complex Subunit 2 antibody
Anti-GINS2 antibody
Anti-PSF2 antibody
Anti-CGI-122 antibody
Anti-DC5 antibody
Anti-HSPC037 antibody

Information sourced from Uniprot.org

Citations

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