Anti-GFI1 antibody [5D7] (STJ98107)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human Gfi-1 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Zinc finger protein Gfi-1 for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5D7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GFI1 |
| Gene ID | 2672 |
| Uniprot ID | GFI1_HUMAN |
| Immunogen | Purified recombinant fragment of human Gfi-1 expressed in E. Coli. |
| Specificity | Gfi-1 Monoclonal Antibody detects endogenous levels of Gfi-1 protein. |
Additional Info
| Function | Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCACATGCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Inhibits SPI1 transcriptional activity at macrophage-specific genes, repressing macrophage differentiation of myeloid progenitor cells and promoting granulocyte commitment. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells. In addition to its role in transcription, acts as a substrate adapter for PRMT1 in the DNA damage response: facilitates the recognition of TP53BP1 and MRE11 substrates by PRMT1, promoting their methylation and the DNA damage response. |
| Protein Name | Zinc Finger Protein Gfi-1Growth Factor Independent Protein 1Zinc Finger Protein 163 |
| Database Links | Reactome: R-HSA-9616222 |
| Cellular Localisation | NucleusColocalizes With Pias3 And Runx1t1 In Nuclear Dots |
| Alternative Antibody Names | Anti-Zinc Finger Protein Gfi-1 antibodyAnti-Growth Factor Independent Protein 1 antibodyAnti-Zinc Finger Protein 163 antibodyAnti-GFI1 antibodyAnti-ZNF163 antibody |
Information sourced from Uniprot.org