• Western blot analysis of lysates from COLO205 cells, using GFAP Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GFAP Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of COS7 cells, using GFAP Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of RAW using GFAP Polyclonal Antibody diluted at 1:2000
  • Western blot analysis of RAT-MUSCLE cells using GFAP Polyclonal Antibody diluted at 1:2000
  • Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

SKU:
STJ93259

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glial fibrillary acidic protein (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFAP
Gene ID: 2670
Uniprot ID: GFAP_HUMAN
Immunogen Region: 11-60 aa
Specificity: GFAP Polyclonal Antibody detects endogenous levels of GFAP protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GFAP at the amino acid range 11-60
Post Translational Modifications Phosphorylated by PKN1.
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Protein Name Glial Fibrillary Acidic Protein
Gfap
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-9613829
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Glial Fibrillary Acidic Protein antibody
Anti-Gfap antibody
Anti-GFAP antibody

Information sourced from Uniprot.org

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