• Immunoprecipitation analysis of 200 Mu g extracts of HT-1080 cells using 3 Mu g GDF15 antibody (STJ23764). Western blot was performed from the immunoprecipitate using GDF15 antibody (STJ23764) at a dilution of 1:1000.
  • Immunofluorescence analysis of BALB-3T3 cells using GDF15 Rabbit polyclonal antibody (STJ23764) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GDF15 in paraffin-embedded human placenta using GDF15 Rabbit polyclonal antibody (STJ23764) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GDF15 Rabbit polyclonal antibody (STJ23764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GDF15 antibody (49-308) (STJ23764)

SKU:
STJ23764

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GDF15 (49-308) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDF15
Gene ID: 9518
Uniprot ID: GDF15_HUMAN
Immunogen Region: 49-308
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-308 of human GDF15 (NP_004855.2).
Immunogen Sequence: EDSRFRELRKRYEDLLTRLR ANQSWEDSNTDLVPAPAVRI LTPEVRLGSGGHLHLRISRA ALPEGLPEASRLHRALFRLS PTASRSWDVTRPLRRQLSLA RPQAPALHLRLSPPPSQSDQ LLAESSSARPQLELHLRPQA ARGRRRARARNGDHCPLGPG RCCRLHTVRASLEDLGWADW VLSPREVQVTMCIGACPSQF RAANMHAQIKTSLHRLKPDT VPAPCCVPASYNPMVLIQK
Tissue Specificity Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level).
Function Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling.
Protein Name Growth/Differentiation Factor 15
Gdf-15
Macrophage Inhibitory Cytokine 1
Mic-1
Nsaid-Activated Gene 1 Protein
Nag-1
Nsaid-Regulated Gene 1 Protein
Nrg-1
Placental Tgf-Beta
Placental Bone Morphogenetic Protein
Prostate Differentiation Factor
Cellular Localisation Secreted
Alternative Antibody Names Anti-Growth/Differentiation Factor 15 antibody
Anti-Gdf-15 antibody
Anti-Macrophage Inhibitory Cytokine 1 antibody
Anti-Mic-1 antibody
Anti-Nsaid-Activated Gene 1 Protein antibody
Anti-Nag-1 antibody
Anti-Nsaid-Regulated Gene 1 Protein antibody
Anti-Nrg-1 antibody
Anti-Placental Tgf-Beta antibody
Anti-Placental Bone Morphogenetic Protein antibody
Anti-Prostate Differentiation Factor antibody
Anti-GDF15 antibody
Anti-MIC1 antibody
Anti-PDF antibody
Anti-PLAB antibody
Anti-PTGFB antibody

Information sourced from Uniprot.org

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