• Western blot analysis of extracts of various cell lines, using GCLM antibody (STJ11101331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GCLM antibody (175-274) [S1MR] (STJ11101331)

SKU:
STJ11101331

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GCLM (175-274) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCLM
Gene ID: 2730
Uniprot ID: GSH0_HUMAN
Immunogen Region: 175-274
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 175-274 of human GCLM (P48507).
Immunogen Sequence: LYQWAQVKPNSNQVNLASCC VMPPDLTAFAKQFDIQLLTH NDPKELLSEASFQEALQESI PDIQAHEWVPLWLLRYSVIV KSRGIIKSKGYILQAKRRGS
Tissue Specificity In all tissues examined. Highest levels in skeletal muscle.
Protein Name Glutamate--Cysteine Ligase Regulatory Subunit
Gcs Light Chain
Gamma-Ecs Regulatory Subunit
Gamma-Glutamylcysteine Synthetase Regulatory Subunit
Glutamate--Cysteine Ligase Modifier Subunit
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-5578999
Reactome: R-HSA-9818027
Alternative Antibody Names Anti-Glutamate--Cysteine Ligase Regulatory Subunit antibody
Anti-Gcs Light Chain antibody
Anti-Gamma-Ecs Regulatory Subunit antibody
Anti-Gamma-Glutamylcysteine Synthetase Regulatory Subunit antibody
Anti-Glutamate--Cysteine Ligase Modifier Subunit antibody
Anti-GCLM antibody
Anti-GLCLR antibody

Information sourced from Uniprot.org

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