| Short Description : | Rabbit polyclonal GCKR (1-300) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GCKR |
| Gene ID: | 2646 |
| Uniprot ID: | GCKR_HUMAN |
| Immunogen Region: | 1-300 |
| Immunogen Sequence: | MPGTKRFQHVIETPEPGKWE LSGYEAAVPITEKSNPLTQD LDKADAENIVRLLGQCDAEI FQEEGQALSTYQRLYSESIL TTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVS FNQLMKGLGQKPLYTYLIAG GDRSVVASREGTEDSALHGI EELKKVAAGKKRVIVIGISV GLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIE DWSSTFRQVAERMQKMQEK |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCKR (NP_001477.2). |
| Tissue Specificity | Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. |
| Function | Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity. |
| Protein Name | Glucokinase Regulatory ProteinGkrpGlucokinase Regulator |
| Database Links | Reactome: R-HSA-170822Reactome: R-HSA-5619107 |
| Cellular Localisation | CytoplasmNucleusMitochondrionUnder Low Glucose ConcentrationsGckr Associates With Gck And The Inactive Complex Is Recruited To The Hepatocyte Nucleus |
| Alternative Antibody Names | Anti-Glucokinase Regulatory Protein antibodyAnti-Gkrp antibodyAnti-Glucokinase Regulator antibodyAnti-GCKR antibody |
Information sourced from Uniprot.org
