• Immunofluorescence analysis of A549 cells using GCA antibody (STJ29166).
  • Western blot analysis of extracts of various cell lines, using GCA antibody (STJ29166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GCA antibody (1-217) (STJ29166)

SKU:
STJ29166

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCA (1-217) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCA
Gene ID: 25801
Uniprot ID: GRAN_HUMAN
Immunogen Region: 1-217
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GCA (NP_036330.1).
Immunogen Sequence: MAYPGYGGGFGNFSIQVPGM QMGQPVPETGPAILLDGYSG PAYSDTYSSAGDSVYTYFSA VAGQDGEVDAEELQRCLTQS GINGTYSPFSLETCRIMIAM LDRDHTGKMGFNAFKELWAA LNAWKENFMTVDQDGSGTVE HHELRQAIGLMGYRLSPQTL TTIVKRYSKNGRIFFDDYVA CCVKLRALTDFFRKRDHLQQ GSANFIYDDFLQGTMAI
Tissue Specificity Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.
Function Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
Protein Name Grancalcin
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Cytoplasmic Granule Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Primarily Cytosolic In The Absence Of Calcium Or Magnesium Ions
Relocates To Granules And Other Membranes In Response To Elevated Calcium And Magnesium Levels
Alternative Antibody Names Anti-Grancalcin antibody
Anti-GCA antibody
Anti-GCL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance