| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GBF1 (1-85) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GBF1 |
| Gene ID: |
8729 |
| Uniprot ID: |
GBF1_HUMAN |
| Immunogen Region: |
1-85 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1). |
| Immunogen Sequence: |
MVDKNIYIIQGEINIVVGAI KRNARWSTHTPLDEERDPLL HSFGHLKEVLNSITELSEIE PNVFLRPFLEVIRSEDTTGP ITGLA |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis. |
| Function | Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway.its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport.the function requires its GEF activity (probably at least in part on ARF4 and ARF5). Has GEF activity towards ARF1. Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP. Required for the assembly of the Golgi apparatus. The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions. May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets.however, this function is under debate. In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex. Plays a role in maintaining mitochondrial morphology. |
| Protein Name | Golgi-Specific Brefeldin A-Resistance Guanine Nucleotide Exchange Factor 1Bfa-Resistant Gef 1 |
| Database Links | Reactome: R-HSA-199992Reactome: R-HSA-5620916Reactome: R-HSA-6807878Reactome: R-HSA-6811434 |
| Cellular Localisation | Golgi ApparatusCis-Golgi NetworkEndoplasmic Reticulum-Golgi Intermediate CompartmentTrans-Golgi NetworkCytoplasmLipid DropletMembranePeripheral Membrane ProteinCycles Rapidly On And Off Early Golgi MembranesStabilized On Membranes When Complexed With Arf1-Gdp And Is Released From Both Arf1 And Membranes After It Catalyzes Gdp Displacement And Arf1 Binds GtpContinuous Cycles Of Recruitment And Dissociation Of Gbf1 To Membranes Are Required For Sustained Arf Activation And Cop I RecruitmentIn Neutrophils Is Translocated From The Golgi To The Leading Edge Upon Gpcr StimulationLocalization To Lipid Droplets Is Questionable |
| Alternative Antibody Names | Anti-Golgi-Specific Brefeldin A-Resistance Guanine Nucleotide Exchange Factor 1 antibodyAnti-Bfa-Resistant Gef 1 antibodyAnti-GBF1 antibodyAnti-KIAA0248 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
