• Immunofluorescence analysis of A549 cells using GATM Rabbit polyclonal antibody (STJ28681). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GATM in paraffin-embedded human liver using GATM Rabbit polyclonal antibody (STJ28681) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GATM Rabbit polyclonal antibody (STJ28681) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GATM antibody (38-423) (STJ28681)

SKU:
STJ28681

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GATM (38-423) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATM
Gene ID: 2628
Uniprot ID: GATM_HUMAN
Immunogen Region: 38-423
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 38-423 of human GATM (NP_001473.1?).
Immunogen Sequence: STQAATASSRNSCAADDKAT EPLPKDCPVSSYNEWDPLEE VIVGRAENACVPPFTIEVKA NTYEKYWPFYQKQGGHYFPK DHLKKAVAEIEEMCNILKTE GVTVRRPDPIDWSLKYKTPD FESTGLYSAMPRDILIVVGN EIIEAPMAWRSRFFEYRAYR SIIKDYFHRGAKWTTAPKPT MADELYNQDYPIHSVEDRHK LAAQGKFVTTEFEPCFDAAD FIRAGRDIFAQRSQVTNYL
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney. Biallelically expressed in placenta and fetal tissues.
Function Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives. Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable).
Protein Name Glycine Amidinotransferase - Mitochondrial
L-Arginine -Glycine Amidinotransferase
Transamidinase
Database Links Reactome: R-HSA-71288
Cellular Localisation Isoform 1: Mitochondrion Inner Membrane
Peripheral Membrane Protein
Intermembrane Side
Probably Attached To The Outer Side Of The Inner Membrane
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Glycine Amidinotransferase - Mitochondrial antibody
Anti-L-Arginine -Glycine Amidinotransferase antibody
Anti-Transamidinase antibody
Anti-GATM antibody
Anti-AGAT antibody

Information sourced from Uniprot.org

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