• Western blot analysis of extracts of various cell lines, using GATA3 antibody (STJ11101740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GATA3 antibody (STJ11101740). Western blot was performed from the immunoprecipitate using GATA3 antibody (STJ11101740) at a dilution of 1:500.
  • Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 rabbit monoclonal antibody antibody (STJ11101740) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-GATA3 antibody (1-200) [S0MR] (STJ11101740)

SKU:
STJ11101740

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GATA3 (1-200) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:100-1:500
ChIP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA3
Gene ID: 2625
Uniprot ID: GATA3_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Immunogen Sequence: MEVTADQPRWVSHHHPAVLN GQHPDTHHPGLSHSYMDAAQ YPLPEEVDVLFNIDGQGNHV PPYYGNSVRATVQRYPPTHH GSQVCRPPLLHGSLPWLDGG KALGSHHTASPWNLSPFSKT SIHHGSPGPLSVYPPASSSS LSGGHASPHLFTFPPTPPKD VSPDPSLSTPGSAGSARQDE KECLKYQVPLPDSMKLESSH
Tissue Specificity T-cells and endothelial cells.
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Protein Name Trans-Acting T-Cell-Specific Transcription Factor Gata-3
Gata-Binding Factor 3
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibody
Anti-Gata-Binding Factor 3 antibody
Anti-GATA3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance