| Host: | Mouse |
| Applications: | ICC/WB |
| Reactivity: | Human/Mouse/Other Mammals/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Galectin-3 (Full-Length) for use in ICC and WB in Human, Mouse, Other Mammals and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 5C21 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | Affinity-purified |
| Dilution Range: | WB 1.4305555555555556ICC 0.73611111111111116 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
| Gene Symbol: | LGALS3 |
| Gene ID: | 3958 |
| Uniprot ID: | LEG3_HUMAN |
| Immunogen Region: | Full-Length |
| Specificity: | Human; mouse; rat; predicted to react with other species due to sequence homology;. |
| Immunogen: | Full length recombinant Galectin-3 expressed in and purified from E. coli. |
| Immunogen Sequence: | Human |
| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. |
| Protein Name | Galectin-3Gal-335 Kda LectinCarbohydrate-Binding Protein 35Cbp 35Galactose-Specific Lectin 3Galactoside-Binding ProteinGalbpIge-Binding ProteinL-31Laminin-Binding ProteinLectin L-29Mac-2 Antigen |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-879415Reactome: R-HSA-8939246Reactome: R-HSA-8941333 |
| Cellular Localisation | CytoplasmNucleusSecretedSecreted By A Non-Classical Secretory Pathway And Associates With The Cell SurfaceCan Be SecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Galectin-3 antibodyAnti-Gal-3 antibodyAnti-35 Kda Lectin antibodyAnti-Carbohydrate-Binding Protein 35 antibodyAnti-Cbp 35 antibodyAnti-Galactose-Specific Lectin 3 antibodyAnti-Galactoside-Binding Protein antibodyAnti-Galbp antibodyAnti-Ige-Binding Protein antibodyAnti-L-31 antibodyAnti-Laminin-Binding Protein antibodyAnti-Lectin L-29 antibodyAnti-Mac-2 Antigen antibodyAnti-LGALS3 antibodyAnti-MAC2 antibody |
Information sourced from Uniprot.org
![Anti-Galectin-3 antibody [9C4] (STJ16100369)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149086/346704/STJ16100369_1__57878.1681998789.jpg?c=1 "Anti-Galectin-3 antibody [9C4] (STJ16100369)")