• Immunofluorescence analysis of HeLa cells using GALC Rabbit polyclonal antibody (STJ111199) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using GALC Rabbit polyclonal antibody (STJ111199) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GALC in paraffin-embedded human kidney using GALC Rabbit polyclonal antibody (STJ111199) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GALC Rabbit polyclonal antibody (STJ111199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GALC antibody (40-300) (STJ111199)

SKU:
STJ111199

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GALC (40-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GALC
Gene ID: 2581
Uniprot ID: GALC_HUMAN
Immunogen Region: 40-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human GALC (NP_000144.2).
Immunogen Sequence: GGAYVLDDSDGLGREFDGIG AVSGGGATSRLLVNYPEPYR SQILDYLFKPNFGASLHILK VEIGGDGQTTDGTEPSHMHY ALDENYFRGYEWWLMKEAKK RNPNITLIGLPWSFPGWLGK GFDWPYVNLQLTAYYVVTWI VGAKRYHDLDIDYIGIWNER SYNANYIKILRKMLNYQGLQ RVKIIASDNLWESISASMLL DAELFKVVDVIGAHYPGTHS AKDAKLTGKKLWSSEDFST
Tissue Specificity Detected in urine. Detected in testis, brain and placenta (at protein level). Detected in kidney and liver.
Function Hydrolyzes the galactose ester bonds of glycolipids such as galactosylceramide and galactosylsphingosine. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
Protein Name Galactocerebrosidase
Galcerase
Galactocerebroside Beta-Galactosidase
Galactosylceramidase
Galactosylceramide Beta-Galactosidase
Database Links Reactome: R-HSA-1660662
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Galactocerebrosidase antibody
Anti-Galcerase antibody
Anti-Galactocerebroside Beta-Galactosidase antibody
Anti-Galactosylceramidase antibody
Anti-Galactosylceramide Beta-Galactosidase antibody
Anti-GALC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance