| Short Description : | Rabbit polyclonal anti-Cyclin-G-associated kinase (101-150 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GAK |
| Gene ID: | 2580 |
| Uniprot ID: | GAK_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human GAK at the amino acid range 101-150 |
| Immunogen Region: | 101-150 aa |
| Specificity: | GAK Polyclonal Antibody detects endogenous levels of GAK protein. |
| Function | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. May play a role in clathrin-mediated endocytosis and intracellular trafficking, and in the dynamics of clathrin assembly/disassembly. |
| Protein Name | Cyclin-G-Associated KinaseDnaj Homolog Subfamily C Member 26 |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmPerinuclear RegionGolgi ApparatusTrans-Golgi NetworkCell JunctionFocal AdhesionCytoplasmic VesicleClathrin-Coated VesicleLocalizes To The Perinuclear Area And To The Trans-Golgi NetworkAlso Seen On The Plasma MembraneProbably At Focal AdhesionsRecruitment To Clathrin-Coated Vesicles Depends On Temporal Variations In Phosphoinositide Composition Of Clathrin-Coated Vesicles |
| Alternative Antibody Names | Anti-Cyclin-G-Associated Kinase antibodyAnti-Dnaj Homolog Subfamily C Member 26 antibodyAnti-GAK antibodyAnti-DNAJC26 antibody |
Information sourced from Uniprot.org
