• Western blot analysis of various lysates using G2E3 Rabbit polyclonal antibody (STJ116620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-G2E3 antibody (1-180) (STJ116620)

SKU:
STJ116620

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-G2E3 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: G2E3
Gene ID: 55632
Uniprot ID: G2E3_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human G2E3 (NP_060239.2).
Immunogen Sequence: MNESKPGDSQNLACVFCRKH DDCPNKYGEKKTKEKWNLTV HYYCLLMSSGIWQRGKEEEG VYGFLIEDIRKEVNRASKLK CCVCKKNGASIGCVAPRCKR SYHFPCGLQRECIFQFTGNF ASFCWDHRPVQIITSNNYRE SLPCTICLEFIEPIPSYNIL RSPCCKNAWFHRDCLQVQAI
Tissue Specificity Predominantly expressed in brain, liver, kidney, testes and ovary.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
Protein Name G2/M Phase-Specific E3 Ubiquitin-Protein Ligase
G2/M Phase-Specific Hect-Type E3 Ubiquitin Transferase
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
In The Nucleus
Delocalizes From The Nucleolus To The Nucleoplasm In Response To Dna Damage
Alternative Antibody Names Anti-G2/M Phase-Specific E3 Ubiquitin-Protein Ligase antibody
Anti-G2/M Phase-Specific Hect-Type E3 Ubiquitin Transferase antibody
Anti-G2E3 antibody
Anti-KIAA1333 antibody

Information sourced from Uniprot.org

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