| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FZR1 (224-493) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FZR1 |
| Gene ID: |
51343 |
| Uniprot ID: |
FZR1_HUMAN |
| Immunogen Region: |
224-493 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 224-493 of human FZR1 (NP_057347.2). |
| Immunogen Sequence: |
DLSVEGDSVTSVGWSERGNL VAVGTHKGFVQIWDAAAGKK LSMLEGHTARVGALAWNAEQ LSSGSRDRMILQRDIRTPPL QSERRLQGHRQEVCGLKWST DHQLLASGGNDNKLLVWNHS SLSPVQQYTEHLAAVKAIAW SPHQHGLLASGGGTADRCIR FWNTLTGQPLQCIDTGSQVC NLAWSKHANELVSTHGYSQN QILVWKYPSLTQVAKLTGHS YRVLYLAMSPDGEAIVTGA |
| Tissue Specificity | Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. |
| Post Translational Modifications | Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159.deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK. Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.leading to its degradation by the proteasome. |
| Function | Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). |
| Protein Name | Fizzy-Related Protein HomologFzrCdc20-Like Protein 1Cdh1/Hct1 HomologHcdh1 |
| Database Links | Reactome: R-HSA-174084Reactome: R-HSA-174113Reactome: R-HSA-174178Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176417Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-69656Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 2: NucleusIsoform 3: Cytoplasm |
| Alternative Antibody Names | Anti-Fizzy-Related Protein Homolog antibodyAnti-Fzr antibodyAnti-Cdc20-Like Protein 1 antibodyAnti-Cdh1/Hct1 Homolog antibodyAnti-Hcdh1 antibodyAnti-FZR1 antibodyAnti-CDH1 antibodyAnti-FYR antibodyAnti-FZR antibodyAnti-KIAA1242 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
