• Western blot analysis of various lysates using FZD8 Rabbit polyclonal antibody (STJ11100346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FZD8 antibody (130-275) (STJ11100346)

SKU:
STJ11100346

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FZD8 (130-275) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FZD8
Gene ID: 8325
Uniprot ID: FZD8_HUMAN
Immunogen Region: 130-275
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-275 of human FZD8 (NP_114072.1).
Immunogen Sequence: PDRMRCDRLPEQGNPDTLCM DYNRTDLTTAAPSPPRRLPP PPPGEQPPSGSGHGRPPGAR PPHRGGGRGGGGGDAAAPPA RGGGGGGKARPPGGGAAPCE PGCQCRAPMVSVSSERHPLY NRVKTGQIANCALPCHNPFF SQDERA
Tissue Specificity Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
Protein Name Frizzled-8
Fz-8
Hfz8
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-4608870
Reactome: R-HSA-4641263
Reactome: R-HSA-5340588
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Golgi Apparatus
Cell Membrane
Colocalizes With Gopc At The Golgi Apparatus
Alternative Antibody Names Anti-Frizzled-8 antibody
Anti-Fz-8 antibody
Anti-Hfz8 antibody
Anti-FZD8 antibody

Information sourced from Uniprot.org

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