Anti-FZD7 antibody (1-260) (STJ23727)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FZD7 (1-260) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FZD7 |
| Gene ID | 8324 |
| Uniprot ID | FZD7_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MRDPGAAAPLSSLGLCALVL ALLGALSAGAGAQPYHGEKG ISVPDHGFCQPISIPLCTDI AYNQTILPNLLGHTNQEDAG LEVHQFYPLVKVQCSPELRF FLCSMYAPVCTVLDQAIPPC RSLCERARQGCEALMNKFGF QWPERLRCENFPVHGAGEIC VGQNTSDGSGGPGGGPTAYP TAPYLPDLPFTALPPGASDG RGRPAFPFSCPRQLKVPPYL GYRFLGERDCGAPCEPGRA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FZD7 (NP_003498.1). |
Additional Info
| Tissue Specificity | High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas. |
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes. Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. |
| Protein Name | Frizzled-7Fz-7Hfz7Fze3 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4086400Reactome: R-HSA-4608870Reactome: R-HSA-9673324 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndosome MembraneAssociated To The Plasma Membrane In The Presence Of Fzd7 And Phosphatidylinositol 45-Bisphosphate (Pip2)Localized In Recycling Endosomes In Other Conditions |
| Alternative Antibody Names | Anti-Frizzled-7 antibodyAnti-Fz-7 antibodyAnti-Hfz7 antibodyAnti-Fze3 antibodyAnti-FZD7 antibody |
Information sourced from Uniprot.org