| Host: |
Mouse |
| Applications: |
IP/WB/IHC-P/ICC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Fyn is suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
FYN-1 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Concentration: |
1 mg/mL |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Gene Symbol: |
FYN |
| Gene ID: |
2534 |
| Uniprot ID: |
FYN_HUMAN |
| Immunogen: |
Bacterially expressed recombinant fragment of human Fyn (aa 7-176). |
| Tissue Specificity | Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. |
| Post Translational Modifications | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1. Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location. |
| Function | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling. |
| Protein Name | Tyrosine-Protein Kinase FynProto-Oncogene SynProto-Oncogene C-FynSrc-Like KinaseSlkP59-Fyn |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1227986Reactome: R-HSA-1257604Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-164944Reactome: R-HSA-202733Reactome: R-HSA-2029481Reactome: R-HSA-210990Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-2682334Reactome: R-HSA-2730905Reactome: R-HSA-373753Reactome: R-HSA-375165Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-389513Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-3928664Reactome: R-HSA-3928665Reactome: R-HSA-399954Reactome: R-HSA-399955Reactome: R-HSA-399956Reactome: R-HSA-418885 P06241-1Reactome: R-HSA-4420097Reactome: R-HSA-5621480Reactome: R-HSA-5621575Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-75892Reactome: R-HSA-8866376Reactome: R-HSA-9032500 P06241-1Reactome: R-HSA-9032759 P06241-1Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9670439Reactome: R-HSA-9680350Reactome: R-HSA-9706374Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmNucleusCell MembranePerikaryonPresent And Active In Lipid RaftsPalmitoylation Is Crucial For Proper Trafficking |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Fyn antibodyAnti-Proto-Oncogene Syn antibodyAnti-Proto-Oncogene C-Fyn antibodyAnti-Src-Like Kinase antibodyAnti-Slk antibodyAnti-P59-Fyn antibodyAnti-FYN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
