• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g FUS antibody (STJ27717). Western blot was performed from the immunoprecipitate using FUS antibody (STJ27717) at a dilution of 1:2000.
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] FUS Rabbit polyclonal antibody (STJ27717) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] FUS Rabbit polyclonal antibody (STJ27717) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and FUS knockout (KO) 293T cells, using [KO Validated] FUS Rabbit polyclonal antibody (STJ27717) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FUS antibody (297-526) (STJ27717)

SKU:
STJ27717

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FUS (297-526) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FUS
Gene ID: 2521
Uniprot ID: FUS_HUMAN
Immunogen Region: 297-526
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
Immunogen Sequence: TIESVADYFKQIGIIKTNKK TGQPMINLYTDRETGKLKGE ATVSFDDPPSAKAAIDWFDG KEFSGNPIKVSFATRRADFN RGGGNGRGGRGRGGPMGRGG YGGGGSGGGGRGGFPSGGGG GGGQQRAGDWKCPNPTCENM NFSWRNECNQCKAPKPDGPG GGPGGSHMGGNYGDDRRGGR GGYDRGGYRGRGGDRGGFRG GRGGGDRGGFGPGKMDSRGE HRQDRRERPY
Tissue Specificity Ubiquitous.
Post Translational Modifications Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine. Phosphorylated in its N-terminal serine residues upon induced DNA damage. ATM and DNA-PK are able to phosphorylate FUS N-terminal region.
Function DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression.this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis.
Protein Name Rna-Binding Protein Fus
75 Kda Dna-Pairing Protein
Oncogene Fus
Oncogene Tls
Pomp75
Translocated In Liposarcoma Protein
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Displays A Punctate Pattern Inside The Nucleus And Is Excluded From Nucleoli
Alternative Antibody Names Anti-Rna-Binding Protein Fus antibody
Anti-75 Kda Dna-Pairing Protein antibody
Anti-Oncogene Fus antibody
Anti-Oncogene Tls antibody
Anti-Pomp75 antibody
Anti-Translocated In Liposarcoma Protein antibody
Anti-FUS antibody
Anti-TLS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance