• Western blot analysis of extracts of various cell lines, using FTSJ3 antibody (STJ112035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-FTSJ3 antibody (608-847) (STJ112035)

SKU:
STJ112035

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FTSJ3 (608-847) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTSJ3
Gene ID: 117246
Uniprot ID: SPB1_HUMAN
Immunogen Region: 608-847
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 608-847 of human FTSJ3 (NP_060117.3).
Immunogen Sequence: EGEEKDGISDSDSSTSSEEE ESWEPLRGKKRSRGPKSDDD GFEIVPIEDPAKHRILDPEG LALGAVIASSKKAKRDLIDN SFNRYTFNEDEGELPEWFVQ EEKQHRIRQLPVGKKEVEHY RKRWREINARPIKKVAEAKA RKKRRMLKRLEQTRKKAEAV VNTVDISEREKVAQLRSLYK KAGLGKEKRHVTYVVAKKGV GRKVRRPAGVRGHFKVVDSR MKKDQRAQQRKEQKKKHKR
Post Translational Modifications Citrullinated by PADI4.
Function RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation. (Microbial infection) In case of infection by HIV-1 virus, recruited to HIV-1 RNA and catalyzes 2'-O-methylation of the viral genome, allowing HIV-1 virus to escape the innate immune system. RNA 2'-O-methylation provides a molecular signature for discrimination of self from non-self and is used by HIV-1 to evade innate immune recognition by IFIH1/MDA5. Mediates methylation of internal residues of HIV-1 RNA, with a strong preference for adenosine. Recruited to HIV-1 RNA via interaction with TARBP2/TRBP.
Protein Name Pre-Rrna 2'-O-Ribose Rna Methyltransferase Ftsj3
Protein Ftsj Homolog 3
Putative Rrna Methyltransferase 3
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Pre-Rrna 2'-O-Ribose Rna Methyltransferase Ftsj3 antibody
Anti-Protein Ftsj Homolog 3 antibody
Anti-Putative Rrna Methyltransferase 3 antibody
Anti-FTSJ3 antibody
Anti-SB92 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance