• Western blot analysis of extracts of various cell lines, using Ferritin Light Chain antibody (STJ26177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Rat liver, using Ferritin Light Chain antibody (STJ26177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using Ferritin Light Chain rabbit polyclonal antibody (STJ26177) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-FTL antibody (1-175) (STJ26177)

SKU:
STJ26177

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FTL (1-175) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTL
Gene ID: 2512
Uniprot ID: FRIL_HUMAN
Immunogen Region: 1-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Immunogen Sequence: MSSQIRQNYSTDVEAAVNSL VNLYLQASYTYLSLGFYFDR DDVALEGVSHFFRELAEEKR EGYERLLKMQNQRGGRALFQ DIKKPAEDEWGKTPDAMKAA MALEKKLNQALLDLHALGSA RTDPHLCDFLETHFLDEEVK LIKKMGDHLTNLHRLGGPEA GLGEYLFERLTLKHD
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Protein Name Ferritin Light Chain
Ferritin L Subunit
Database Links Reactome: R-HSA-3000480
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-917937
Alternative Antibody Names Anti-Ferritin Light Chain antibody
Anti-Ferritin L Subunit antibody
Anti-FTL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance