||WB, IHC-P, IF, ELISA
||Rabbit polyclonal anti-Frizzled-6 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000
||Frizzled-6 polyclonal antibody detects endogenous levels of Frizzled-6 protein.
||Synthesized peptide derived from human Frizzled-6
||90-170 aa, Internal
| Post Translational Modifications || Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function || Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. |
| Protein Name || Frizzled-6Fz-6Hfz6 |
| Database Links || Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4086400Reactome: R-HSA-4641263Reactome: R-HSA-5340588 |
| Cellular Localisation || MembraneMulti-Pass Membrane ProteinCell MembraneCell SurfaceApical Cell MembraneCytoplasmic Vesicle MembraneEndoplasmic Reticulum MembraneColocalizes With Fzd3 At The Apical Face Of CellsLocalizes To The Endoplasmic Reticulum Membrane In The Presence Of Lmbr1l |
| Alternative Antibody Names || Anti-Frizzled-6 antibodyAnti-Fz-6 antibodyAnti-Hfz6 antibodyAnti-FZD6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance