Anti-Frizzled-5/8 antibody (31-80 aa) (STJ93148)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human FZD8 at the amino acid range 31-80
General Information
| Short Description | Rabbit polyclonal anti-Frizzled-5 and Frizzled-8 (31-80 aa) for use in WB, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FZD5FZD8 |
| Gene ID | 78558325 |
| Uniprot ID | FZD5_HUMANFZD8_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human FZD8 at the amino acid range 31-80 |
| Immunogen Region | 31-80 aa |
| Specificity | Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein. |
Additional Info
| Function | Receptor for Wnt proteins. Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro).the in vivo situation may be different since not all of these are known to be coexpressed. In neurons, activation of WNT7A promotes formation of synapses. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization. |
| Protein Name | Frizzled-5Fz-5Hfz5Fze5 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4608870Reactome: R-HSA-4641262Reactome: R-HSA-4641263Reactome: R-HSA-5140745Reactome: R-HSA-5340588 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCell JunctionSynapsePerikaryonCell ProjectionDendriteAxonLocalized At The Plasma Membrane And Also Found At The Golgi |
| Alternative Antibody Names | Anti-Frizzled-5 antibodyAnti-Fz-5 antibodyAnti-Hfz5 antibodyAnti-Fze5 antibodyAnti-FZD5 antibodyAnti-C2orf31 antibody |
Information sourced from Uniprot.org