• Western blot analysis of the lysates from HeLa cells using FZD3 antibody.
  • Western blot analysis of lysates from A549 and RAW264.7 cells, using FZD3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of RAW264.7 cells using Frizzled-3 Polyclonal Antibody
  • Immunofluorescence analysis of COS7 cells, using FZD3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Frizzled-3 Polyclonal Antibody

Anti-Frizzled-3 antibody (STJ93142)

SKU:
STJ93142
Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human,Mouse
Short Description: Rabbit polyclonal primary antibody against Frizzled-3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Specificity: Frizzled-3 polyclonal antibody detects endogenous levels of Frizzled-3 protein.
Uniprot ID: FZD3_HUMAN
Gene ID: 7976
Gene Symbol: FZD3
Immunogen: Synthesized peptide derived from human Frizzled-3
Immunogen Region: 110-190 aa, Internal
Tissue Specificity Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Required for the development of major fiber tracts in the central nervous system, including: the anterior commissure, the corpus callosum, the thalamocortical, corticothalamic and nigrostriatal tracts, the corticospinal tract, the fasciculus retroflexus, the mammillothalamic tract, the medial lemniscus, and ascending fiber tracts from the spinal cord to the brain. In the peripheral nervous system, controls axon growth in distinct populations of cranial and spinal motor neurons, including the facial branchimotor nerve, the hypoglossal nerve, the phrenic nerve, and motor nerves innervating dorsal limbs. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner.
Database Links Reactome: R-HSA-373080
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cell Surface
Apical Cell Membrane
Colocalizes With Fzd6 At The Apical Face Of The Cell
Protein Name Frizzled-3
Fz-3
Hfz3
Alternative Names Anti-Frizzled-3 antibody
Anti-Fz-3 antibody
Anti-Hfz3 antibody
Anti-FZD3 antibody
6 months standard