• Western blot analysis of lysates from HeLa cells, using FZD2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HepG2 cells using FZD2 antibody.
  • Immunofluorescence analysis of MCF7 cells, using FZD2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Frizzled-2 antibody (STJ93141)

SKU:
STJ93141

Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-Frizzled-2 antibody is suitable for use in Western Blot, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Specificity: Frizzled-2 polyclonal antibody detects endogenous levels of Frizzled-2 protein.
Uniprot ID: FZD2_HUMAN
Gene ID: 2535
Gene Symbol: FZD2
Immunogen: Synthesized peptide derived from human Frizzled-2
Immunogen Region: 180-260 aa, Internal
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling.
Protein Name Frizzled-2
Fz-2
Hfz2
Fze2
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-373080
Reactome: R-HSA-4086398
Reactome: R-HSA-4608870
Reactome: R-HSA-4641262
Reactome: R-HSA-5140745
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Frizzled-2 antibody
Anti-Fz-2 antibody
Anti-Hfz2 antibody
Anti-Fze2 antibody
Anti-FZD2 antibody

Information sourced from Uniprot.org

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