• Western blot analysis of lysates from COS7 cells, using FZD10 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of COS-7 cells using Frizzled-10 Polyclonal Antibody

Anti-Frizzled-10 antibody (STJ93140)

SKU:
STJ93140
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human,Mouse,Monkey
Short Description: Rabbit polyclonal primary antibody against Frizzled-10.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Specificity: Frizzled-10 polyclonal antibody detects endogenous levels of Frizzled-10 protein.
Uniprot ID: FZD10_HUMAN
Gene ID: 11211
Gene Symbol: FZD10
Immunogen: Synthesized peptide derived from human Frizzled-10
Immunogen Region: 110-190 aa, Internal
Tissue Specificity Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord.very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).
Database Links Reactome: R-HSA-373080
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Protein Name Frizzled-10
Fz-10
Hfz10
Fze7
Cd Antigen Cd350
Alternative Names Anti-Frizzled-10 antibody
Anti-Fz-10 antibody
Anti-Hfz10 antibody
Anti-Fze7 antibody
Anti-Cd Antigen Cd350 antibody
Anti-FZD10 antibody
6 months standard