||WB, IF, ELISA
||Rabbit polyclonal anti-Frizzled-1 antibody is suitable for use in Western Blot, Immunofluorescence and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IF 1:200-1:1000ELISA 1:5000
||Frizzled-1 polyclonal antibody detects endogenous levels of Frizzled-1 protein.
||Synthesized peptide derived from human Frizzled-1
||30-110 aa, N-terminal
| Post Translational Modifications || Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function || Receptor for Wnt proteins. Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B. Contradictory results showing activation by WNT7B have been described for mouse. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. |
| Protein Name || Frizzled-1Fz-1Hfz1Fze1 |
| Database Links || Reactome: R-HSA-201681Reactome: R-HSA-373080Reactome: R-HSA-4086400Reactome: R-HSA-4608870Reactome: R-HSA-4641262 |
| Cellular Localisation || Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names || Anti-Frizzled-1 antibodyAnti-Fz-1 antibodyAnti-Hfz1 antibodyAnti-Fze1 antibodyAnti-FZD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance