• Western blot analysis of lysates from HeLa and COLO205 cells, using FZD1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HT-29 cells using FZD1 antibody.
  • Western blot analysis of various cells using Frizzled-1 Polyclonal Antibody

Anti-Frizzled-1 antibody (STJ93138)

SKU:
STJ93138

Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal anti-Frizzled-1 antibody is suitable for use in Western Blot, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Specificity: Frizzled-1 polyclonal antibody detects endogenous levels of Frizzled-1 protein.
Uniprot ID: FZD1_HUMAN
Gene ID: 8321
Gene Symbol: FZD1
Immunogen: Synthesized peptide derived from human Frizzled-1
Immunogen Region: 30-110 aa, N-terminal
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B. Contradictory results showing activation by WNT7B have been described for mouse. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium.
Protein Name Frizzled-1
Fz-1
Hfz1
Fze1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-373080
Reactome: R-HSA-4086400
Reactome: R-HSA-4608870
Reactome: R-HSA-4641262
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Frizzled-1 antibody
Anti-Fz-1 antibody
Anti-Hfz1 antibody
Anti-Fze1 antibody
Anti-FZD1 antibody

Information sourced from Uniprot.org

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