• Immunohistochemistry analysis of paraffin-embedded human lymphocyto tissue, showing cytoplasmic and nuclear localization with DAB staining using FoxP3 monoclonal antibody.
  • Confocal immunofluorescence analysis of PANC-1 cells using FoxP3 monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using FoxP3 monoclonal antibody against truncated Foxp3 recombinant (1) and HEK293 cell lysate (2).

Anti-FoxP3 antibody (STJ98088)

SKU:
STJ98088
Current Stock:
Host: Mouse
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse
Short Description: Mouse monoclonal primary antibody against FoxP3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 2A11G9/2A11C2
Conjugation: Unconjugated
Formulation: PBS containing 0.03% sodium azide.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Specificity: FoxP3 monoclonal antibody detects endogenous levels of FoxP3 protein.
Uniprot ID: FOXP3_HUMAN
Gene ID: 50943
Gene Symbol: FOXP3
Immunogen: Purified recombinant fragment of human FoxP3 expressed in E. Coli.
Post Translational Modifications Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. Deubiquitinated by USP7 leading to increase in protein stability. Phosphorylation at Ser-418 regulates its transcriptional repressor activity and consequently, regulatory T-cells (Treg) suppressive function. Dephosphorylated at Ser-418 by protein phosphatase 1 (PP1) in Treg cells derived from patients with rheumatoid arthritis. Phosphorylation by CDK2 negatively regulates its transcriptional activity and protein stability. Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. Undergoes proteolytic cleavage in activated regulatory T-cells (Treg), and can be cleaved at either the N- or C-terminal site, or at both sites.
Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Database Links Reactome: R-HSA-8877330
Cellular Localisation Nucleus
Cytoplasm
Predominantly Expressed In The Cytoplasm In Activated Conventional T-Cells Whereas Predominantly Expressed In The Nucleus In Regulatory T-Cells (Treg)
The 41 Kda Form Derived By Proteolytic Processing Is Found Exclusively In The Chromatin Fraction Of Activated Treg Cells
Protein Name Forkhead Box Protein P3
Scurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form
Alternative Names Anti-Forkhead Box Protein P3 antibody
Anti-Scurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form antibody
Anti-FOXP3 antibody
Anti-IPEX antibody
Anti-JM2 antibody

Information sourced from Uniprot.org

6 months standard