• Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200
  • Western blot analysis of K562 3T3 lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-FLT4 antibody (640-700 aa) (STJ98712)

SKU:
STJ98712

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vascular endothelial growth factor receptor 3 (640-700 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:500-200
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLT4
Gene ID: 2324
Uniprot ID: VGFR3_HUMAN
Immunogen Region: 640-700 aa
Specificity: The antibody detects endogenous Flt-4
Immunogen: Synthetic peptide from the human protein at the amino acid range 640-700
Post Translational Modifications Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H(2)O(2) is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC at Tyr-830, Tyr-833, Tyr-853, Tyr-1063, Tyr-1333, and Tyr-1337.
Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades.isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
Protein Name Vascular Endothelial Growth Factor Receptor 3
Vegfr-3
Fms-Like Tyrosine Kinase 4
Flt-4
Tyrosine-Protein Kinase Receptor Flt4
Database Links Reactome: R-HSA-195399
Reactome: R-HSA-9013695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Nucleus
Ligand-Mediated Autophosphorylation Leads To Rapid Internalization
Isoform 1: Cell Membrane
Isoform 2: Cell Membrane
Isoform 3: Secreted
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor Receptor 3 antibody
Anti-Vegfr-3 antibody
Anti-Fms-Like Tyrosine Kinase 4 antibody
Anti-Flt-4 antibody
Anti-Tyrosine-Protein Kinase Receptor Flt4 antibody
Anti-FLT4 antibody
Anti-VEGFR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance