![Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389175/STJ27976_3__74293.1713158812.jpg?c=1 "Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389176/STJ27976_4__36753.1713158813.jpg?c=1 "Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389177/STJ27976_5__16396.1713158814.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389178/STJ27976_6__26072.1713158814.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-FLOT1 antibody (155-427) (STJ27976)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FLOT1 (155-427) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FLOT1 |
| Gene ID | 10211 |
| Uniprot ID | FLOT1_HUMAN |
| Immunogen Region | 155-427 |
| Immunogen Sequence | HDDQDYLHSLGKARTAQVQK DARIGEAEAKRDAGIREAKA KQEKVSAQYLSEIEMAKAQR DYELKKAAYDIEVNTRRAQA DLAYQLQVAKTKQQIEEQRV QVQVVERAQQVAVQEQEIAR REKELEARVRKPAEAERYKL ERLAEAEKSQLIMQAEAEAA SVRMRGEAEAFAIGARARAE AEQMAKKAEAFQLYQEAAQL DMLLEKLPQVAEEISGPLTS ANKITLVSSGSGTMGAAKV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 155-427 of human Flotillin 1 (NP_005794.1). |
Additional Info
| Function | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. |
| Protein Name | Flotillin-1 |
| Database Links | Reactome: R-HSA-5213460Reactome: R-HSA-5675482Reactome: R-HSA-8849932Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinEndosomeMembraneCaveolaMelanosomeMembrane RaftIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvMembrane-Associated Protein Of Caveola |
| Alternative Antibody Names | Anti-Flotillin-1 antibodyAnti-FLOT1 antibody |
Information sourced from Uniprot.org