• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Flotillin 1 antibody (STJ27976). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (STJ27976) at a dilution of 1:1000.
  • Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FLOT1 antibody (155-427) (STJ27976)

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STJ27976

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FLOT1 (155-427) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLOT1
Gene ID: 10211
Uniprot ID: FLOT1_HUMAN
Immunogen Region: 155-427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-427 of human Flotillin 1 (NP_005794.1).
Immunogen Sequence: HDDQDYLHSLGKARTAQVQK DARIGEAEAKRDAGIREAKA KQEKVSAQYLSEIEMAKAQR DYELKKAAYDIEVNTRRAQA DLAYQLQVAKTKQQIEEQRV QVQVVERAQQVAVQEQEIAR REKELEARVRKPAEAERYKL ERLAEAEKSQLIMQAEAEAA SVRMRGEAEAFAIGARARAE AEQMAKKAEAFQLYQEAAQL DMLLEKLPQVAEEISGPLTS ANKITLVSSGSGTMGAAKV
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Protein Name Flotillin-1
Database Links Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-8849932
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Endosome
Membrane
Caveola
Melanosome
Membrane Raft
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Membrane-Associated Protein Of Caveola
Alternative Antibody Names Anti-Flotillin-1 antibody
Anti-FLOT1 antibody

Information sourced from Uniprot.org

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