• Western blot analysis of extracts of various cell lines, using FKBP38/FKBP8 rabbit monoclonal antibody (STJ11102358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of NIH/3T3 cells using FKBP38/FKBP8 Rabbit monoclonal antibody (STJ11102358, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of extracts of various cell lines, using FKBP38/FKBP8 rabbit monoclonal antibody (STJ11102358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using FKBP38/FKBP8 Rabbit monoclonal antibody (STJ11102358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using FKBP38/FKBP8 rabbit monoclonal antibody (STJ11102358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using FKBP38/FKBP8 Rabbit monoclonal antibody (STJ11102358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of NIH-3T3 cells using FKBP38/FKBP8 rabbit monoclonal antibody (STJ11102358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-FKBP8 antibody (1-100) [S8MR] (STJ11102358)

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STJ11102358

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FKBP38 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FKBP8
Gene ID: 23770
Uniprot ID: FKBP8_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FKBP38/FKBP8 (Q14318).
Immunogen Sequence: MASCAEPSEPSAPLPAGVPP LEDFEVLDGVEDAEGEEEEE EEEEEEDDLSELPPLEDMGQ PPAEEAEQPGALAREFLAAM EPEPAPAPAPEEWLDILGNG
Tissue Specificity Widely expressed. Highest levels seen in the brain. Highly abundant in the retina.
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. Involved in the inhibition of viral infection by influenza A viruses (IAV).
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase Fkbp8
Ppiase Fkbp8
38 Kda Fk506-Binding Protein
38 Kda Fkbp
Fkbp-38
Hfkbp38
Fk506-Binding Protein 8
Fkbp-8
Fkbpr38
Rotamase
Database Links Reactome: R-HSA-5689880
Cellular Localisation Mitochondrion
Mitochondrion Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
Isoform 1: Mitochondrion Membrane
Isoform 3: Mitochondrion Membrane
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase Fkbp8 antibody
Anti-Ppiase Fkbp8 antibody
Anti-38 Kda Fk506-Binding Protein antibody
Anti-38 Kda Fkbp antibody
Anti-Fkbp-38 antibody
Anti-Hfkbp38 antibody
Anti-Fk506-Binding Protein 8 antibody
Anti-Fkbp-8 antibody
Anti-Fkbpr38 antibody
Anti-Rotamase antibody
Anti-FKBP8 antibody
Anti-FKBP38 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance