| Short Description : | Rabbit monoclonal FHL1 (1-100) antibody for use in WB, IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FHL1 |
| Gene ID: | 2273 |
| Uniprot ID: | FHL1_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen Sequence: | MAEKFDCHYCRDPLQGKKYV QKDGHHCCLKCFDKFCANTC VECRKPIGADSKEVHYKNRF WHDTCFRCAKCLHPLANETF VAKDNKILCNKCTTREDSPK |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FHL1 (Q13642). |
| Tissue Specificity | Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle. |
| Function | May have an involvement in muscle development or hypertrophy. |
| Protein Name | Four And A Half Lim Domains Protein 1Fhl-1Skeletal Muscle Lim-Protein 1SlimSlim-1 |
| Database Links | |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 3: CytoplasmNucleusIsoform 2: NucleusCytoplasmCytosolPredominantly Nuclear In Myoblasts But Is Cytosolic In Differentiated Myotubes |
| Alternative Antibody Names | Anti-Four And A Half Lim Domains Protein 1 antibodyAnti-Fhl-1 antibodyAnti-Skeletal Muscle Lim-Protein 1 antibodyAnti-Slim antibodyAnti-Slim-1 antibodyAnti-FHL1 antibodyAnti-SLIM1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90216/359234/STJ11101303_1__60787.1713123659.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cell pools, using [KD Validated] GPX4 Rabbit monoclonal antibody (STJ11101303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
