• Immunohistochemistry analysis of paraffin-embedded human colon using FHL1 Rabbit monoclonal antibody (STJ11102223) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of SH-SY5Y cells, using FHL1 Rabbit monoclonal antibody (STJ11102223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FHL1 antibody (1-100) [S3MR] (STJ11102223)

SKU:
STJ11102223

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FHL1 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FHL1
Gene ID: 2273
Uniprot ID: FHL1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FHL1 (Q13642).
Immunogen Sequence: MAEKFDCHYCRDPLQGKKYV QKDGHHCCLKCFDKFCANTC VECRKPIGADSKEVHYKNRF WHDTCFRCAKCLHPLANETF VAKDNKILCNKCTTREDSPK
Tissue Specificity Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.
Function May have an involvement in muscle development or hypertrophy.
Protein Name Four And A Half Lim Domains Protein 1
Fhl-1
Skeletal Muscle Lim-Protein 1
Slim
Slim-1
Cellular Localisation Isoform 1: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Isoform 2: Nucleus
Cytoplasm
Cytosol
Predominantly Nuclear In Myoblasts But Is Cytosolic In Differentiated Myotubes
Alternative Antibody Names Anti-Four And A Half Lim Domains Protein 1 antibody
Anti-Fhl-1 antibody
Anti-Skeletal Muscle Lim-Protein 1 antibody
Anti-Slim antibody
Anti-Slim-1 antibody
Anti-FHL1 antibody
Anti-SLIM1 antibody

Information sourced from Uniprot.org

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