• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from A549 cells, using FHIT Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of various cells using Fhit Polyclonal Antibody

Anti-FHIT antibody (81-130 aa) (STJ93069)

SKU:
STJ93069

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bis5 adenosyl-triphosphatase (81-130 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FHIT
Gene ID: 2272
Uniprot ID: FHIT_HUMAN
Immunogen Region: 81-130 aa
Specificity: Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human FHIT at the amino acid range 81-130
Post Translational Modifications Phosphorylation at Tyr-114 by SRC is required for induction of apoptosis.
Function Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate. Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Protein Name Bis(5'-Adenosyl-Triphosphatase
Ap3a Hydrolase
Ap3aase
Adenosine 5'-Monophosphoramidase Fhit
Adenylylsulfatase
Adenylylsulfate-Ammonia Adenylyltransferase
Diadenosine 5' -5'''-P1 -P3-Triphosphate Hydrolase
Dinucleosidetriphosphatase
Fragile Histidine Triad Protein
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Alternative Antibody Names Anti-Bis(5'-Adenosyl-Triphosphatase antibody
Anti-Ap3a Hydrolase antibody
Anti-Ap3aase antibody
Anti-Adenosine 5'-Monophosphoramidase Fhit antibody
Anti-Adenylylsulfatase antibody
Anti-Adenylylsulfate-Ammonia Adenylyltransferase antibody
Anti-Diadenosine 5' -5'''-P1 -P3-Triphosphate Hydrolase antibody
Anti-Dinucleosidetriphosphatase antibody
Anti-Fragile Histidine Triad Protein antibody
Anti-FHIT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance