• Immunohistochemistry analysis of paraffin-embedded rat liver using FGF21 Rabbit monoclonal antibody (STJ11101967) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using FGF21 Rabbit monoclonal antibody (STJ11101967) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using FGF21 Rabbit monoclonal antibody (STJ11101967) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using FGF21 Rabbit monoclonal antibody (STJ11101967) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FGF21 antibody (29-209) [S7MR] (STJ11101967)

SKU:
STJ11101967

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FGF21 (29-209) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGF21
Gene ID: 26291
Uniprot ID: FGF21_HUMAN
Immunogen Region: 29-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-209 of human FGF21 (NP_061986.1).
Immunogen Sequence: HPIPDSSPLLQFGGQVRQRY LYTDDAQQTEAHLEIREDGT VGGAADQSPESLLQLKALKP GVIQILGVKTSRFLCQRPDG ALYGSLHFDPEACSFRELLL EDGYNVYQSEAHGLPLHLPG NKSPHRDPAPRGPARFLPLP GLPPALPEPPGILAPQPPDV GSSDPLSMVGPSQGRSPSYA S
Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Protein Name Fibroblast Growth Factor 21
Fgf-21
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-8963889
Cellular Localisation Secreted
Alternative Antibody Names Anti-Fibroblast Growth Factor 21 antibody
Anti-Fgf-21 antibody
Anti-FGF21 antibody
Anti-UNQ3115 antibody
Anti-PRO10196 antibody

Information sourced from Uniprot.org

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