• Western blot analysis of lysates from COLO205 cells, using FEN1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of various cells using FEN-1 Polyclonal Antibody diluted at 1：500

Anti-FEN1 antibody (86-135 aa) (STJ93054)

SKU:
STJ93054

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Flap endonuclease 1 (86-135 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FEN1
Gene ID: 2237
Uniprot ID: FEN1_HUMAN
Immunogen Region: 86-135 aa
Specificity: FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human FEN1 at the amino acid range 86-135
Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Protein Name Flap Endonuclease 1
Fen-1
Dnase Iv
Flap Structure-Specific Endonuclease 1
Maturation Factor 1
Mf1
Hfen-1
Database Links Reactome: R-HSA-110362
Reactome: R-HSA-162594
Reactome: R-HSA-174437
Reactome: R-HSA-5651801
Reactome: R-HSA-5685939
Reactome: R-HSA-69166
Cellular Localisation Isoform 1: Nucleus
Nucleolus
Nucleus
Nucleoplasm
Resides Mostly In The Nucleoli And Relocalizes To The Nucleoplasm Upon Dna Damage
Isoform Fenmit: Mitochondrion
Alternative Antibody Names Anti-Flap Endonuclease 1 antibody
Anti-Fen-1 antibody
Anti-Dnase Iv antibody
Anti-Flap Structure-Specific Endonuclease 1 antibody
Anti-Maturation Factor 1 antibody
Anti-Mf1 antibody
Anti-Hfen-1 antibody
Anti-FEN1 antibody
Anti-RAD2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance