• Western blot analysis of extracts of HL-60 cells, using FCGRT antibody (STJ111283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of THP-1, using FCGRT rabbit polyclonal antibody (STJ111283) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A-549 cells using FCGRT rabbit polyclonal antibody (STJ111283) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-FCGRT antibody (27-290) (STJ111283)

SKU:
STJ111283

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FCGRT (27-290) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FCGRT
Gene ID: 2217
Uniprot ID: FCGRN_HUMAN
Immunogen Region: 27-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-290 of human FcRn (NP_004098.1).
Immunogen Sequence: HLSLLYHLTAVSSPAPGTPA FWVSGWLGPQQYLSYNSLRG EAEPCGAWVWENQVSWYWEK ETTDLRIKEKLFLEAFKALG GKGPYTLQGLLGCELGPDNT SVPTAKFALNGEEFMNFDLK QGTWGGDWPEALAISQRWQQ QDKAANKELTFLLFSCPHRL REHLERGRGNLEWKEPPSMR LKARPSSPGFSVLTCSAFSF YPPELQLRFLRNGLAAGTGQ GDFGPNSDGSFHASSSLTV
Tissue Specificity Expressed in full-term placenta, heart, lung, liver, muscle, kidney, pancreas, and both fetal and adult small intestine.
Function Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation. In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB. (Microbial infection) Acts as an uncoating receptor for a panel of echoviruses including Echovirus 5, 6, 7, 9, 11, 13, 25 and 29.
Protein Name Igg Receptor Fcrn Large Subunit P51
Fcrn
Igg Fc Fragment Receptor Transporter Alpha Chain
Neonatal Fc Receptor
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Alternative Antibody Names Anti-Igg Receptor Fcrn Large Subunit P51 antibody
Anti-Fcrn antibody
Anti-Igg Fc Fragment Receptor Transporter Alpha Chain antibody
Anti-Neonatal Fc Receptor antibody
Anti-FCGRT antibody
Anti-FCRN antibody

Information sourced from Uniprot.org

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