• Immunohistochemistry analysis of Fbx32/FBXO32 in paraffin-embedded rat heart using Fbx32/FBOX32 Rabbit monoclonal antibody (STJ11101932) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Fbx32/FBXO32 Rabbit monoclonal antibody (STJ11101932) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FBXO32 antibody (1-120) [S2MR] (STJ11101932)

SKU:
STJ11101932

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Fbx32 (1-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO32
Gene ID: 114907
Uniprot ID: FBX32_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Fbx32/FBOX32 (Q969P5).
Immunogen Sequence: MPFLGQDWRSPGQNWVKTAD GWKRFLDEKSGSFVSDLSSY CNKEVYNKENLFNSLNYDVA AKKRKKDMLNSKTKTQYFHQ EKWIYVHKGSTKERHGYCTL GEAFNRLDFSTAILDSRRFN
Tissue Specificity Specifically expressed in cardiac and skeletal muscle.
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Protein Name F-Box Only Protein 32
Atrogin-1
Muscle Atrophy F-Box Protein
Mafbx
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-9615017
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between Cytoplasm And The Nucleus
Alternative Antibody Names Anti-F-Box Only Protein 32 antibody
Anti-Atrogin-1 antibody
Anti-Muscle Atrophy F-Box Protein antibody
Anti-Mafbx antibody
Anti-FBXO32 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance