• Western blot analysis of extracts of mouse thymus, using FANCL antibody (STJ28895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FANCL antibody (1-255) (STJ28895)

SKU:
STJ28895

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FANCL (1-255) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FANCL
Gene ID: 55120
Uniprot ID: FANCL_HUMAN
Immunogen Region: 1-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human FANCL (NP_060532.2).
Immunogen Sequence: MAVTEASLLRQCPLLLPQNR SKTVYEGFISAQGRDFHLRI VLPEDLQLKNARLLCSWQLR TILSGYHRIVQQRMQHSPDL MSFMMELKMLLEVALKNRQE LYALPPPPQFYSSLIEEIGT LGWDKLVYADTCFSTIKLKA EDASGREHLITLKLKAKYPA ESPDYFVDFPVPFCASWTPQ SSLISIYSQFLAAIESLKAF WDVMDEIDEKTWVLEPEKPP RSATARRIALGNNVSINIE
Post Translational Modifications The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
Function Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
Protein Name E3 Ubiquitin-Protein Ligase Fancl
Fanconi Anemia Group L Protein
Fanconi Anemia-Associated Polypeptide Of 43 Kda
Faap43
Ring-Type E3 Ubiquitin Transferase Fancl
Database Links Reactome: R-HSA-6783310
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Fancl antibody
Anti-Fanconi Anemia Group L Protein antibody
Anti-Fanconi Anemia-Associated Polypeptide Of 43 Kda antibody
Anti-Faap43 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Fancl antibody
Anti-FANCL antibody
Anti-PHF9 antibody

Information sourced from Uniprot.org

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