Anti-Factor H antibody [R09-7D4] (STJA0036499)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Factor H
General Information
| Short Description | Rabbit monoclonal anti-Factor H for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R09-7D4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CFH |
| Gene ID | 3075 |
| Uniprot ID | CFAH_HUMAN |
| Immunogen | A synthesized peptide derived from human Factor H |
Additional Info
| Tissue Specificity | Expressed in the retinal pigment epithelium (at protein level). CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells. |
| Post Translational Modifications | Sulfated on tyrosine residues. According to a report, Asn-217 is not glycosylated. Another study observed glycosylation at this position. |
| Function | Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination. |
| Protein Name | Complement Factor HH Factor 1 |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | Secreted(Microbial Infection) In The Mosquito MidgutLocalizes To PFalciparum (Nf54 Strain) Macrogamete And Young Zygote Cell Membranes |
| Alternative Antibody Names | Anti-Complement Factor H antibodyAnti-H Factor 1 antibodyAnti-CFH antibodyAnti-HF antibodyAnti-HF1 antibodyAnti-HF2 antibody |
Information sourced from Uniprot.org