Anti-FA2H antibody (95-170) (STJ115813)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FA2H (95-170) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FA2H |
| Gene ID | 79152 |
| Uniprot ID | FA2H_HUMAN |
| Immunogen Region | 95-170 |
| Immunogen Sequence | NEPVALEETQKTDPAMEPRF KVVDWDKDLVDWRKPLLWQV GHLGEKYDEWVHQPVTRPIR LFHSDLIEGLSKTVWY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 95-170 of human FA2H (NP_077282.3). |
Additional Info
| Tissue Specificity | Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney. |
| Function | Catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. FA2H is stereospecific for the production of (R)-2-hydroxy fatty acids. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins. |
| Protein Name | Fatty Acid 2-HydroxylaseFatty Acid Alpha-HydroxylaseFatty Acid Hydroxylase Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-1660661 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Fatty Acid 2-Hydroxylase antibodyAnti-Fatty Acid Alpha-Hydroxylase antibodyAnti-Fatty Acid Hydroxylase Domain-Containing Protein 1 antibodyAnti-FA2H antibodyAnti-FAAH antibodyAnti-FAXDC1 antibody |
Information sourced from Uniprot.org