• Western blot analysis of extracts of various cell lines, using F2RL3 antibody (STJ110769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using F2RL3 antibody (STJ110769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-F2RL3 antibody (20-150) (STJ110769)

SKU:
STJ110769

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F2RL3 (20-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F2RL3
Gene ID: 9002
Uniprot ID: PAR4_HUMAN
Immunogen Region: 20-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human F2RL3 (NP_003941.2).
Immunogen Sequence: TQTPSVYDESGSTGGGDDST PSILPAPRGYPGQVCANDSD TLELPDSSRALLLGWVPTRL VPALYGLVLVVGLPANGLAL WVLATQAPRLPSTMLLMNLA AADLLLALALPPRIAYHLRG QRWPFGEAACR
Tissue Specificity Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.
Post Translational Modifications A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Function Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.
Protein Name Proteinase-Activated Receptor 4
Par-4
Coagulation Factor Ii Receptor-Like 3
Thrombin Receptor-Like 3
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Reactome: R-HSA-456926
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Proteinase-Activated Receptor 4 antibody
Anti-Par-4 antibody
Anti-Coagulation Factor Ii Receptor-Like 3 antibody
Anti-Thrombin Receptor-Like 3 antibody
Anti-F2RL3 antibody
Anti-PAR4 antibody

Information sourced from Uniprot.org

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