Anti-F2R antibody (42-425) (STJ27608)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal F2R (42-425) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | F2R |
| Gene ID | 2149 |
| Uniprot ID | PAR1_HUMAN |
| Immunogen Region | 42-425 |
| Immunogen Sequence | SFLLRNPNDKYEPFWEDEEK NESGLTEYRLVSINKSSPLQ KQLPAFISEDASGYLTSSWL TLFVPSVYT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 42-110 of human F2R (NP_001983.2). |
Additional Info
| Tissue Specificity | Platelets and vascular endothelial cells. |
| Post Translational Modifications | Proteolytic cleavage by thrombin generates a new N-terminus that functions as a tethered ligand. Also proteolytically cleaved by cathepsin CTSG. Cleavage at 41-Arg-|-Ser-42 by CTSG results in receptor activation while cleavage at 55-Phe-|-Trp-56 results in inhibition of receptor activation. Phosphorylated in the C-terminal tail.probably mediating desensitization prior to the uncoupling and internalization of the receptor. |
| Function | High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores. The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores. In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. May play a role in platelets activation and in vascular development. Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells. |
| Protein Name | Proteinase-Activated Receptor 1Par-1Coagulation Factor Ii ReceptorThrombin Receptor |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-456926 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Proteinase-Activated Receptor 1 antibodyAnti-Par-1 antibodyAnti-Coagulation Factor Ii Receptor antibodyAnti-Thrombin Receptor antibodyAnti-F2R antibodyAnti-CF2R antibodyAnti-PAR1 antibodyAnti-TR antibody |
Information sourced from Uniprot.org