| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EYA1 (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EYA1 |
| Gene ID: |
2138 |
| Uniprot ID: |
EYA1_HUMAN |
| Immunogen Region: |
200-300 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human EYA1 (NP_000494.2). |
| Immunogen Sequence: |
NSLTNSSGFNSSQQDYPSYP SFGQGQYAQYYNSSPYPAHY MTSSNTSPTTPSTNATYQLQ EPPSGITSQAVTDPTAEYST IHSPSTPIKDSDSDRLRRGS D |
| Tissue Specificity | In the embryo, highly expressed in kidney with lower levels in brain. Weakly expressed in lung. In the adult, highly expressed in heart and skeletal muscle. Weakly expressed in brain and liver. No expression in eye or kidney. |
| Post Translational Modifications | Sumoylated with SUMO1. |
| Function | Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development.in this it is functionally redundant with EYA2. |
| Protein Name | Eyes Absent Homolog 1 |
| Database Links | Reactome: R-HSA-5693565 |
| Cellular Localisation | CytoplasmNucleusLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Eyes Absent Homolog 1 antibodyAnti-EYA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
