Anti-EXT2 antibody (C-Term) (STJ71937)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-EXT2 (C-Term) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments in lysates of cell line HEK293 gave no specific signal but low background (at antibody concentration up to 1µg/ml). |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | EXT2 |
| Gene ID | 2132 |
| Uniprot ID | EXT2_HUMAN |
| Accession Number | NP_000392.2; NP_997005.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | PLKVVEHRADPVL |
| Specificity | This antibody is expected to recognize isoform 1 and 2 (NP_000392.2; NP_997005.1). |
Additional Info
| Post Translational Modifications | N-glycosylated at Asn-637. A soluble form is generated by proteolytic processing. |
| Function | Glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone. Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity. Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling. |
| Protein Name | Exostosin-2Exostosin Glycosyltransferase 2Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-AcetylglucosaminyltransferaseHeparan Sulfate Co-Polymerase Subunit Ext1Multiple Exostoses Protein 2 |
| Database Links | Reactome: R-HSA-2022928Reactome: R-HSA-3656237Reactome: R-HSA-3656253 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinGolgi ApparatusCis-Golgi Network MembraneEndoplasmic Reticulum MembraneSecretedThe Active Heparan Sulfate Polymerase Complex Composed Of Ext1 And Ext2 Is Localized To The Golgi ApparatusIf Both Proteins Are Individually Detected In The Endoplasmic ReticulumThe Formation Of The Complex Promotes Their Transport To The Golgi |
| Alternative Antibody Names | Anti-Exostosin-2 antibodyAnti-Exostosin Glycosyltransferase 2 antibodyAnti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibodyAnti-Heparan Sulfate Co-Polymerase Subunit Ext1 antibodyAnti-Multiple Exostoses Protein 2 antibodyAnti-EXT2 antibody |
Information sourced from Uniprot.org